Background color in Prototype mode does not change

I want to change the color of my background in prototype mode.

Whenever I change the background color in prototype settings, the background color in prototype mode does not change on play.

My steps:
I change the color in the prototype settings to blue, and the color in preview mode change to blue as well. But when I click on Play icon to start the prototype, the background color stays white. See attachments.

What am I doing wrong?

And I don’t know the reason why the alpha channel is not supported in Presentation mode.

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The alpha channel is not supported, that is why my confusion. I have set the opacity to 100% manually. Thank you very much!